Preparative-Scale Electrophoresis on Acrylamide Gel
نویسندگان
چکیده
منابع مشابه
Continuous preparative gel electrophoresis.
Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
متن کاملThin-layer acrylamide gel electrophoresis.
A thin-layer acrylamide gel electrophoresis technique suitable for analysis of serum or urine proteins is described in detail. The method gives sharp differentiation of protein fractions and is particularly suitable for the analysis of biological solutions containing proteins in low concentration.
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By THOMAS G. FERRIS, ROBERT E. EASTERLING AND RICHARD E. BUDD T HE VARIOUS types of hemoglobins have been identified and reported by means of zone electrophoresis using supporting media such as filter paper,’ starch,2 agar,3 cellulose acetate,4 and combinations of these media. Recently a synthetic acrylamide gel ( Cyanogum 41#{176} ) has been investigated and found to have characteristics which...
متن کاملA new apparatus for preparative gel electrophoresis.
paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi ca...
متن کاملPurification of horse serum cholinesterase by preparative polyacrylamide gel electrophoresis.
Preparative polyacrylamide gel electrophoresis has been used in the final stages of purification to prepare horse serum cholinesterase which was at least 9.5% pure, as judged by analytical polyacrylamide gel electrophoresis. The starting material, already purified about 109-fold by the Strelitz method, was purified an additional 89-fold by the procedure. The method involved Sephadex G-ZOO and S...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1966
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(66)87796-2